《植物生理学报》 2017, 53(4): 513-520

间歇浸没式液体培养技术及其在莓类果实植物微扩繁中的应用

石琨^1,2, 郑彩霞^3,*, 张运涛^1,2,*, 钟传飞^1,2, 王桂霞^1,2, 董静^1,2, 常琳琳^1,2, 孙健^1,2

¹北京市农林科学院林业果树研究所, 北京100093; ²北京市草莓工程技术研究中心, 北京100093; ³北京林业大学生物科学与技术学院, 北京100083

通信作者：郑彩霞；E-mail: zhengcx@bjfu.edu.cn; zhytao1963@126.com

摘　要：

建立高效种苗组织培养快速繁殖技术对于保障莓类果实植物优质高产、满足日益增长的市场需求具有重要意义。本文综述间歇浸没式液体培养技术的发展及其在植物微扩繁中的应用, 分析和评价该技术在草莓、树莓、蓝莓等几种重要莓类植物繁殖中的研究现状, 并展望了其在莓类植物研究应用领域的发展前景。

关键词：间歇浸没; 液体培养; 莓类植物; 植物微扩繁

收稿：2017-02-09   修定：2017-03-28

资助：国家重点研发计划“政府间国际科技创新合作”重点专项(2016YFE0112400)和北京市科技专项(Z161100005016111)。

The technology of temporary immersion in liquid culture and its application in micropropagation of berry plants

SHI Kun^1,2, ZHENG Cai-Xia^3,*, ZHANG Yun-Tao^1,2,*, ZHONG Chuan-Fei^1,2, WANG Gui-Xia^1,2, DONG Jing^1,2, CHANG Lin-Lin^1,2, SUN Jian^1,2

¹Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100093, China; ²Beijing Engineering Research Center for Strawberry, Beijing 100093, China; ³College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, China

Corresponding author: ZHENG Cai-Xia; E-mail: zhengcx@bjfu.edu.cn; zhytao1963@126.com

Abstract:

The establishment of a high efficiency micropropagation system for plant seedlings is critically important for high quality and yield production of berry plants to meet the increasing market demand. The development of temporary immersion technology in liquid culture and its application in plant micropropagation was reviewed. The research of the temporary immersion culture for propagation in several important berry plants such as strawberry, raspberry and blueberry was discussed and evaluated, and the development prospect of this technique in the research and application fields of berry plants was projected.

Key words: temporary immersion; liquid culture; berry plants; plant micropropagation